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Epub Sep 6. Tsikas D 1. The identification of nitrite in saliva has been the first analytical application of this diazotization reaction in For a century, the Griess reaction has been exclusively used to identify analytically bacterial infection in the urogenital tract, i. The Griess reaction is specific for nitrite. Analysis of nitrate by this reaction requires chemical or enzymatic reduction of nitrate to nitrite prior to the diazotization reaction.
Today, we know dozens of assays based on the Griess reaction. In principle, every laboratory in this area uses its own Griess assay. The simplest Griess assay is performed in batch commonly as originally reported by Griess.
Because of the recognition of numerous interferences in the analysis of nitrite and nitrate in biological fluids and of the desire to analyze these anions simultaneously, the Griess reaction has been repeatedly modified and automated.
The present article gives an overview of the currently available assays of nitrite and nitrate in biological fluids based on the Griess reaction. Special emphasis is given to human plasma and urine, to quantitative aspects, as well as to particular analytical and pre-analytical factors and problems that may be associated with and affect the quantitative analysis of nitrite and nitrate in these matrices by assays based on the Griess reaction.
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Life[ edit ] After he finished at an agricultural private school, he joined the Hessian cavalry , but left the military shortly after. He started his studies at the University of Jena in , but changed to the University of Marburg in During his student life he was several times sentenced to the Karzer campus jail and was also banned from the city for one year, during which time he listened to lectures of Justus Liebig at the Ludwig Maximilian University of Munich. After most of the family possession had been spent, he had to start working at the chemical factory of Oehler in Offenbach am Main in This was only possible after the recommendation of Hermann Kolbe , who was head of the chemistry department in Marburg.