It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics Provides useful, detailed, easy-to-follow, step-by-step protocols Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates Cites over more than references keyed to concepts covered in the book Uses more than figures to illustrate bioconjugate reagents, their reactions, and applications Suggests sources for all key reagents.
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Hermanson, is the essential reference that every biotechnology scientist needs. Read More This third edition of Bioconjugate Techniques outshines the two previous editions in breadth, depth, and usefulness.
Moreover, several hundred full-color illustrations throughout this third edition of Bioconjugate Techniques notably improve presentation of the material compared to previous editions. New Chapters Indeed, the new Chapter 1 comprehensively introduces readers who are unfamiliar with bioconjugation to the subject. Chapter 1 should be required reading for every student or professional scientist who is new to the subject of bioconjugation. The chapter begins with a broad look at affinity chromatography and immobilization chemistry.
It then discusses the types of solid phase supports used in this type of chemistry. From there, the chapter moves through an extensive, thoughtful discussion of how to activate, modify, and use these valuable, increasingly important chromatography supports. Useful Recipes and Procedures Throughout Bioconjugate Techniques, Greg Hermanson provides tested, proven recipes for carrying out successful bioconjugation techniques.
Readers can succeed with these protocols without possessing a doctorate in chemistry. They are simple, well-written procedures. Text discussions around the recipes explain possible problems and how to troubleshoot failed reactions.